diff --git a/api/python/CMakeLists.txt b/api/python/CMakeLists.txt
index 265161f4a3..44167505df 100644
--- a/api/python/CMakeLists.txt
+++ b/api/python/CMakeLists.txt
@@ -23,8 +23,8 @@ endif()
 if (LIEF_EXTERNAL_NANOBINDS)
   find_package(nanobind REQUIRED)
 else()
-  set(NANOBIND_VERSION 2.2.0.r27.g070f0ce)
-  set(NANOBIND_SHA256 SHA256=7dc0ad8b2f4baff427b6e829b029c422d80d27833732561478b7d262f15ac394)
+  set(NANOBIND_VERSION 2.4.0.r9.g81da6e9)
+  set(NANOBIND_SHA256 SHA256=8c319ad628dd092f98d345cc92e4b670c8538cc01ff8dde9be186987e7fe703f)
   set(NANOBIND_URL "${THIRD_PARTY_DIRECTORY}/nanobind-${NANOBIND_VERSION}.zip"
       CACHE STRING "URL to the Nanobind")
   FetchContent_Declare(nanobind
diff --git a/api/python/src/pyIOStream.cpp b/api/python/src/pyIOStream.cpp
index 8825ef93ba..98f2042970 100644
--- a/api/python/src/pyIOStream.cpp
+++ b/api/python/src/pyIOStream.cpp
@@ -29,7 +29,7 @@ result<PyIOStream> PyIOStream::from_python(nb::object object) {
   const nb::module_ mod_io = nb::module_::import_("io");
   const nb::object IOBase = mod_io.attr("IOBase");
 
-  if (!isinstance(object, IOBase)) {
+  if (!nb::isinstance(object, IOBase)) {
     logging::log(logging::LEVEL::ERR,
         "The provided io object does not sub-class io.IOBase");
     return make_error_code(lief_errors::read_error);
diff --git a/api/python/src/pyutils.hpp b/api/python/src/pyutils.hpp
index 9d1b53760f..ba04d8044c 100644
--- a/api/python/src/pyutils.hpp
+++ b/api/python/src/pyutils.hpp
@@ -9,13 +9,6 @@
 namespace nb = nanobind;
 
 namespace LIEF::py {
-inline bool isinstance(nb::handle obj, nb::handle type) {
-    const auto result = PyObject_IsInstance(obj.ptr(), type.ptr());
-    if (result == -1) {
-      nb::detail::raise_python_error();
-    }
-    return result != 0;
-}
 
 inline std::string type2str(nb::object obj) {
   auto pytype = nb::steal<nb::str>(nb::detail::nb_inst_name(obj.ptr()));
diff --git a/doc/sphinx/changelog.rst b/doc/sphinx/changelog.rst
index 4f38915f18..c554f244fe 100644
--- a/doc/sphinx/changelog.rst
+++ b/doc/sphinx/changelog.rst
@@ -198,7 +198,7 @@
 
 :Python Bindings:
 
-  * Upgrade nanobind from ``1.8.0`` to ``2.2.0``
+  * Upgrade nanobind from ``1.8.0`` to ``2.4.0``
   * ``*.pyi`` stubs are now generated by nanobind (replacing mypy's stugen)
 
 :Dependencies:
diff --git a/third-party/nanobind-2.2.0.r27.g070f0ce.zip b/third-party/nanobind-2.4.0.r9.g81da6e9.zip
similarity index 90%
rename from third-party/nanobind-2.2.0.r27.g070f0ce.zip
rename to third-party/nanobind-2.4.0.r9.g81da6e9.zip
index 68c0db2221..803d33bf88 100644
Binary files a/third-party/nanobind-2.2.0.r27.g070f0ce.zip and b/third-party/nanobind-2.4.0.r9.g81da6e9.zip differ