images/simple-plasmid-construction.svg

<?xml version="1.0" encoding="UTF-8" standalone="no"?>
<!DOCTYPE svg PUBLIC "-//W3C//DTD SVG 1.1//EN" "http://www.w3.org/Graphics/SVG/1.1/DTD/svg11.dtd">
<svg xmlns="http://www.w3.org/2000/svg" xmlns:dc="http://purl.org/dc/elements/1.1/" version="1.1" xmlns:xl="http://www.w3.org/1999/xlink" viewBox="34.5 297.5 826.5 1400" width="826.5" height="1400">
  <defs>
    <font-face font-family="Helvetica Neue" font-size="16" panose-1="2 0 5 3 0 0 0 2 0 4" units-per-em="1000" underline-position="-100" underline-thickness="50" slope="0" x-height="517" cap-height="714" ascent="951.9958" descent="-212.99744" font-weight="400">
      <font-face-src>
        <font-face-name name="HelveticaNeue"/>
      </font-face-src>
    </font-face>
    <font-face font-family="Helvetica Neue" font-size="14" panose-1="2 0 5 3 0 0 0 2 0 4" units-per-em="1000" underline-position="-100" underline-thickness="50" slope="0" x-height="517" cap-height="714" ascent="951.9958" descent="-212.99744" font-weight="400">
      <font-face-src>
        <font-face-name name="HelveticaNeue"/>
      </font-face-src>
    </font-face>
    <marker orient="auto" overflow="visible" markerUnits="strokeWidth" id="FilledArrow_Marker" stroke-linejoin="miter" stroke-miterlimit="10" viewBox="-1 -4 10 8" markerWidth="10" markerHeight="8" color="black">
      <g>
        <path d="M 8 0 L 0 -3 L 0 3 Z" fill="currentColor" stroke="currentColor" stroke-width="1"/>
      </g>
    </marker>
    <font-face font-family="Helvetica Neue" font-size="24" panose-1="2 0 5 3 0 0 0 2 0 4" units-per-em="1000" underline-position="-100" underline-thickness="50" slope="0" x-height="517" cap-height="714" ascent="951.9958" descent="-212.99744" font-weight="400">
      <font-face-src>
        <font-face-name name="HelveticaNeue"/>
      </font-face-src>
    </font-face>
  </defs>
  <metadata> Produced by OmniGraffle 7.10.1 
    <dc:date>2019-03-14 17:07:01 +0000</dc:date>
  </metadata>
  <g id="Canvas_1" stroke="none" fill-opacity="1" stroke-opacity="1" stroke-dasharray="none" fill="none">
    <title>Simple Plasmid Construction</title>
    <g id="Canvas_1: Layer 1">
      <title>Layer 1</title>
      <g id="Graphic_51">
        <path d="M 85 298 L 851.5 298 C 856.4706 298 860.5 302.02944 860.5 307 L 860.5 1688 C 860.5 1692.9706 856.4706 1697 851.5 1697 L 85 1697 C 80.02944 1697 76 1692.9706 76 1688 L 76 307 C 76 302.02944 80.02944 298 85 298 Z" fill="#eee"/>
        <path d="M 85 298 L 851.5 298 C 856.4706 298 860.5 302.02944 860.5 307 L 860.5 1688 C 860.5 1692.9706 856.4706 1697 851.5 1697 L 85 1697 C 80.02944 1697 76 1692.9706 76 1688 L 76 307 C 76 302.02944 80.02944 298 85 298 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_3">
        <path d="M 413.2138 481 L 521.2138 481 C 526.18436 481 530.2138 485.02944 530.2138 490 L 530.2138 544 C 530.2138 548.97056 526.18436 553 521.2138 553 L 413.2138 553 C 408.24323 553 404.2138 548.97056 404.2138 544 L 404.2138 490 C 404.2138 485.02944 408.24323 481 413.2138 481 Z" fill="white"/>
        <path d="M 413.2138 481 L 521.2138 481 C 526.18436 481 530.2138 485.02944 530.2138 490 L 530.2138 544 C 530.2138 548.97056 526.18436 553 521.2138 553 L 413.2138 553 C 408.24323 553 404.2138 548.97056 404.2138 544 L 404.2138 490 C 404.2138 485.02944 408.24323 481 413.2138 481 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 498.552)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="19.624" y="15">Make PCR </tspan>
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="23.768" y="33.448">Fragment</tspan>
        </text>
      </g>
      <g id="Graphic_4">
        <rect x="35" y="403" width="144" height="48" fill="white"/>
        <rect x="35" y="403" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(40 410.608)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="16.838" y="13">Forward Primer: </tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="22.662" y="29.392">Primer Aliquot</tspan>
        </text>
      </g>
      <g id="Graphic_5">
        <rect x="35" y="487" width="144" height="48" fill="white"/>
        <rect x="35" y="487" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(40 494.608)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="17.223" y="13">Reverse Primer: </tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="22.662" y="29.392">Primer Aliquot </tspan>
        </text>
      </g>
      <g id="Graphic_6">
        <rect x="35" y="571" width="144" height="48" fill="white"/>
        <rect x="35" y="571" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(40 586.5)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="38.601" y="13">Template</tspan>
        </text>
      </g>
      <g id="Line_7">
        <line x1="179" y1="427" x2="394.71335" y2="490.21585" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_8">
        <line x1="179" y1="511" x2="394.3173" y2="516.73634" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_9">
        <line x1="179" y1="595" x2="394.71335" y2="531.78415" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_10">
        <rect x="395.2138" y="589" width="144" height="48" fill="white"/>
        <rect x="395.2138" y="589" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(400.2138 604.5)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="6.205" y="13">Fragment: Stripwell</tspan>
        </text>
      </g>
      <g id="Line_11">
        <line x1="467.2138" y1="553" x2="467.2138" y2="579.1" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_12">
        <path d="M 413.2138 679 L 521.2138 679 C 526.18436 679 530.2138 683.0294 530.2138 688 L 530.2138 742 C 530.2138 746.9706 526.18436 751 521.2138 751 L 413.2138 751 C 408.24323 751 404.2138 746.9706 404.2138 742 L 404.2138 688 C 404.2138 683.0294 408.24323 679 413.2138 679 Z" fill="white"/>
        <path d="M 413.2138 679 L 521.2138 679 C 526.18436 679 530.2138 683.0294 530.2138 688 L 530.2138 742 C 530.2138 746.9706 526.18436 751 521.2138 751 L 413.2138 751 C 408.24323 751 404.2138 746.9706 404.2138 742 L 404.2138 688 C 404.2138 683.0294 408.24323 679 413.2138 679 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 705)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="29.256" y="15">Run Gel</tspan>
        </text>
      </g>
      <g id="Line_13">
        <line x1="467.2138" y1="637" x2="467.2138" y2="669.1" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_14">
        <path d="M 652 481 L 760 481 C 764.9706 481 769 485.02944 769 490 L 769 544 C 769 548.97056 764.9706 553 760 553 L 652 553 C 647.0294 553 643 548.97056 643 544 L 643 490 C 643 485.02944 647.0294 481 652 481 Z" fill="white"/>
        <path d="M 652 481 L 760 481 C 764.9706 481 769 485.02944 769 490 L 769 544 C 769 548.97056 764.9706 553 760 553 L 652 553 C 647.0294 553 643 548.97056 643 544 L 643 490 C 643 485.02944 647.0294 481 652 481 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(648 507)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="26.744" y="15">Pour Gel</tspan>
        </text>
      </g>
      <g id="Graphic_15">
        <rect x="634" y="589" width="144" height="48" fill="white"/>
        <rect x="634" y="589" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 604.5)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="28.633" y="13">Agarose Gel</tspan>
        </text>
      </g>
      <g id="Line_16">
        <line x1="706" y1="553" x2="706" y2="579.1" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_17">
        <line x1="634" y1="625" x2="538.1279" y2="697.0522" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_18">
        <rect x="634" y="735" width="144" height="48" fill="white"/>
        <rect x="634" y="735" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 742.608)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="7.36" y="13">Fragment: Agarose </tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="56.374" y="29.392">Gel</tspan>
        </text>
      </g>
      <g id="Line_19">
        <line x1="530.2138" y1="727" x2="624.27885" y2="745.1267" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_20">
        <path d="M 413.2138 787 L 521.2138 787 C 526.18436 787 530.2138 791.0294 530.2138 796 L 530.2138 850 C 530.2138 854.9706 526.18436 859 521.2138 859 L 413.2138 859 C 408.24323 859 404.2138 854.9706 404.2138 850 L 404.2138 796 C 404.2138 791.0294 408.24323 787 413.2138 787 Z" fill="white"/>
        <path d="M 413.2138 787 L 521.2138 787 C 526.18436 787 530.2138 791.0294 530.2138 796 L 530.2138 850 C 530.2138 854.9706 526.18436 859 521.2138 859 L 413.2138 859 C 408.24323 859 404.2138 854.9706 404.2138 850 L 404.2138 796 C 404.2138 791.0294 408.24323 787 413.2138 787 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 804.552)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="18.304" y="15">Extract Gel </tspan>
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="40.672" y="33.448">Slice</tspan>
        </text>
      </g>
      <g id="Graphic_21">
        <path d="M 413.2138 895 L 521.2138 895 C 526.18436 895 530.2138 899.0294 530.2138 904 L 530.2138 958 C 530.2138 962.9706 526.18436 967 521.2138 967 L 413.2138 967 C 408.24323 967 404.2138 962.9706 404.2138 958 L 404.2138 904 C 404.2138 899.0294 408.24323 895 413.2138 895 Z" fill="white"/>
        <path d="M 413.2138 895 L 521.2138 895 C 526.18436 895 530.2138 899.0294 530.2138 904 L 530.2138 958 C 530.2138 962.9706 526.18436 967 521.2138 967 L 413.2138 967 C 408.24323 967 404.2138 962.9706 404.2138 958 L 404.2138 904 C 404.2138 899.0294 408.24323 895 413.2138 895 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 921)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="3.64" y="15">Purify Gel Slice</tspan>
        </text>
      </g>
      <g id="Graphic_22">
        <rect x="634" y="844.2857" width="144" height="48" fill="white"/>
        <rect x="634" y="844.2857" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 851.8937)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="35.101" y="13">Fragment:</tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="39.266" y="29.392">Gel Slice</tspan>
        </text>
      </g>
      <g id="Line_23">
        <line x1="634" y1="771" x2="539.45146" y2="807.4397" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_24">
        <line x1="530.2138" y1="835" x2="624.3019" y2="854.2967" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_25">
        <line x1="634" y1="880.2857" x2="539.4895" y2="915.54" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_26">
        <rect x="634" y="953.5714" width="144" height="48" fill="white"/>
        <rect x="634" y="953.5714" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 961.1794)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="35.101" y="13">Fragment:</tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="16.95" y="29.392">Fragment Stock</tspan>
        </text>
      </g>
      <g id="Line_27">
        <line x1="530.2138" y1="943" x2="624.3261" y2="963.4676" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_28">
        <path d="M 413.2138 1003 L 521.2138 1003 C 526.18436 1003 530.2138 1007.0294 530.2138 1012 L 530.2138 1066 C 530.2138 1070.9706 526.18436 1075 521.2138 1075 L 413.2138 1075 C 408.24323 1075 404.2138 1070.9706 404.2138 1066 L 404.2138 1012 C 404.2138 1007.0294 408.24323 1003 413.2138 1003 Z" fill="white"/>
        <path d="M 413.2138 1003 L 521.2138 1003 C 526.18436 1003 530.2138 1007.0294 530.2138 1012 L 530.2138 1066 C 530.2138 1070.9706 526.18436 1075 521.2138 1075 L 413.2138 1075 C 408.24323 1075 404.2138 1070.9706 404.2138 1066 L 404.2138 1012 C 404.2138 1007.0294 408.24323 1003 413.2138 1003 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 1020.552)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="22.88" y="15">Assemble </tspan>
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="29.4" y="33.448">Plasmid</tspan>
        </text>
      </g>
      <g id="Graphic_29">
        <path d="M 413.2138 1111 L 521.2138 1111 C 526.18436 1111 530.2138 1115.0294 530.2138 1120 L 530.2138 1174 C 530.2138 1178.9706 526.18436 1183 521.2138 1183 L 413.2138 1183 C 408.24323 1183 404.2138 1178.9706 404.2138 1174 L 404.2138 1120 C 404.2138 1115.0294 408.24323 1111 413.2138 1111 Z" fill="white"/>
        <path d="M 413.2138 1111 L 521.2138 1111 C 526.18436 1111 530.2138 1115.0294 530.2138 1120 L 530.2138 1174 C 530.2138 1178.9706 526.18436 1183 521.2138 1183 L 413.2138 1183 C 408.24323 1183 404.2138 1178.9706 404.2138 1174 L 404.2138 1120 C 404.2138 1115.0294 408.24323 1111 413.2138 1111 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 1137)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="2.44" y="15">Transform Cells</tspan>
        </text>
      </g>
      <g id="Graphic_30">
        <path d="M 413.2138 1219 L 521.2138 1219 C 526.18436 1219 530.2138 1223.0294 530.2138 1228 L 530.2138 1282 C 530.2138 1286.9706 526.18436 1291 521.2138 1291 L 413.2138 1291 C 408.24323 1291 404.2138 1286.9706 404.2138 1282 L 404.2138 1228 C 404.2138 1223.0294 408.24323 1219 413.2138 1219 Z" fill="white"/>
        <path d="M 413.2138 1219 L 521.2138 1219 C 526.18436 1219 530.2138 1223.0294 530.2138 1228 L 530.2138 1282 C 530.2138 1286.9706 526.18436 1291 521.2138 1291 L 413.2138 1291 C 408.24323 1291 404.2138 1286.9706 404.2138 1282 L 404.2138 1228 C 404.2138 1223.0294 408.24323 1219 413.2138 1219 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 1227.328)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="39.928" y="15">Plate </tspan>
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="13.248" y="33.448">Transformed </tspan>
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="40.376" y="51.895996">Cells</tspan>
        </text>
      </g>
      <g id="Graphic_31">
        <path d="M 413.2138 1327 L 521.2138 1327 C 526.18436 1327 530.2138 1331.0294 530.2138 1336 L 530.2138 1390 C 530.2138 1394.9706 526.18436 1399 521.2138 1399 L 413.2138 1399 C 408.24323 1399 404.2138 1394.9706 404.2138 1390 L 404.2138 1336 C 404.2138 1331.0294 408.24323 1327 413.2138 1327 Z" fill="white"/>
        <path d="M 413.2138 1327 L 521.2138 1327 C 526.18436 1327 530.2138 1331.0294 530.2138 1336 L 530.2138 1390 C 530.2138 1394.9706 526.18436 1399 521.2138 1399 L 413.2138 1399 C 408.24323 1399 404.2138 1394.9706 404.2138 1390 L 404.2138 1336 C 404.2138 1331.0294 408.24323 1327 413.2138 1327 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 1353)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="14.736" y="15">Check Plate</tspan>
        </text>
      </g>
      <g id="Graphic_32">
        <path d="M 413.2138 1435 L 521.2138 1435 C 526.18436 1435 530.2138 1439.0294 530.2138 1444 L 530.2138 1498 C 530.2138 1502.9706 526.18436 1507 521.2138 1507 L 413.2138 1507 C 408.24323 1507 404.2138 1502.9706 404.2138 1498 L 404.2138 1444 C 404.2138 1439.0294 408.24323 1435 413.2138 1435 Z" fill="white"/>
        <path d="M 413.2138 1435 L 521.2138 1435 C 526.18436 1435 530.2138 1439.0294 530.2138 1444 L 530.2138 1498 C 530.2138 1502.9706 526.18436 1507 521.2138 1507 L 413.2138 1507 C 408.24323 1507 404.2138 1502.9706 404.2138 1498 L 404.2138 1444 C 404.2138 1439.0294 408.24323 1435 413.2138 1435 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 1452.552)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="1.096" y="15">Make Overnight </tspan>
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="16.064" y="33.448">Suspension</tspan>
        </text>
      </g>
      <g id="Graphic_33">
        <path d="M 413.2138 1543 L 521.2138 1543 C 526.18436 1543 530.2138 1547.0294 530.2138 1552 L 530.2138 1606 C 530.2138 1610.9706 526.18436 1615 521.2138 1615 L 413.2138 1615 C 408.24323 1615 404.2138 1610.9706 404.2138 1606 L 404.2138 1552 C 404.2138 1547.0294 408.24323 1543 413.2138 1543 Z" fill="white"/>
        <path d="M 413.2138 1543 L 521.2138 1543 C 526.18436 1543 530.2138 1547.0294 530.2138 1552 L 530.2138 1606 C 530.2138 1610.9706 526.18436 1615 521.2138 1615 L 413.2138 1615 C 408.24323 1615 404.2138 1610.9706 404.2138 1606 L 404.2138 1552 C 404.2138 1547.0294 408.24323 1543 413.2138 1543 Z" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(409.2138 1569)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="16" font-weight="400" fill="black" x="4.792" y="15">Make Miniprep</tspan>
        </text>
      </g>
      <g id="Graphic_34">
        <rect x="634" y="1062.8571" width="144" height="48" fill="white"/>
        <rect x="634" y="1062.8571" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 1070.4651)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="40.029" y="13">Plasmid:</tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="21.878" y="29.392">Plasmid Stock</tspan>
        </text>
      </g>
      <g id="Graphic_35">
        <rect x="634" y="1172.1429" width="144" height="48" fill="white"/>
        <rect x="634" y="1172.1429" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 1179.7509)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="40.029" y="13">Plasmid:</tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="3.986" y="29.392">Transformed Aliquot</tspan>
        </text>
      </g>
      <g id="Line_36">
        <line x1="530.2138" y1="1051" x2="624.3516" y2="1072.6393" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_37">
        <line x1="634" y1="1098.8571" x2="539.5631" y2="1131.7442" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_38">
        <line x1="530.2138" y1="1159" x2="624.3783" y2="1181.812" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_39">
        <line x1="634" y1="1208.1429" x2="539.59863" y2="1239.848" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_40">
        <rect x="634" y="1281.4286" width="144" height="48" fill="white"/>
        <rect x="634" y="1281.4286" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 1296.9286)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="24.216" y="13">Plasmid:Plate</tspan>
        </text>
      </g>
      <g id="Line_41">
        <line x1="530.2138" y1="1267" x2="624.4062" y2="1290.9856" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_42">
        <line x1="634" y1="1317.4286" x2="539.63327" y2="1347.9531" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_43">
        <rect x="634" y="1390.7143" width="144" height="48" fill="white"/>
        <rect x="634" y="1390.7143" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 1406.2143)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="41.975" y="13">Plasmid</tspan>
        </text>
      </g>
      <g id="Line_44">
        <line x1="530.2138" y1="1375" x2="624.43515" y2="1400.1602" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_45">
        <line x1="634" y1="1426.7143" x2="539.66696" y2="1456.0593" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_46">
        <rect x="634" y="1500" width="144" height="48" fill="white"/>
        <rect x="634" y="1500" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(639 1507.608)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="40.029" y="13">Plasmid:</tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="36.403" y="29.392">Overnight</tspan>
        </text>
      </g>
      <g id="Line_47">
        <line x1="530.2138" y1="1483" x2="624.4652" y2="1509.3358" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_48">
        <rect x="35" y="1601" width="144" height="48" fill="white"/>
        <rect x="35" y="1601" width="144" height="48" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        <text transform="translate(40 1608.608)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="40.029" y="13">Plasmid:</tspan>
          <tspan font-family="Helvetica Neue" font-size="14" font-weight="400" fill="black" x="21.878" y="29.392">Plasmid Stock</tspan>
        </text>
      </g>
      <g id="Line_49">
        <line x1="634" y1="1536" x2="539.6997" y2="1564.1667" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_50">
        <line x1="404.2138" y1="1591" x2="188.8531" y2="1612.0375" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_52">
        <line x1="634" y1="989.5714" x2="539.5267" y2="1023.6415" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Graphic_55">
        <text transform="translate(93 316)" fill="black">
          <tspan font-family="Helvetica Neue" font-size="24" font-weight="400" fill="black" x="11.158" y="23">Simple Plasmid Construction</tspan>
        </text>
      </g>
      <g id="Graphic_56">
        <circle cx="584" cy="388" r="9.00001438111709" fill="black"/>
        <circle cx="584" cy="388" r="9.00001438111709" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Group_57">
        <g id="Graphic_59">
          <circle cx="467.2138" cy="1660" r="9.00001438111712" fill="white"/>
          <circle cx="467.2138" cy="1660" r="9.00001438111712" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        </g>
        <g id="Graphic_58">
          <circle cx="467.2138" cy="1660" r="6.50001038636238" fill="black"/>
          <circle cx="467.2138" cy="1660" r="6.50001038636238" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
        </g>
      </g>
      <g id="Graphic_60">
        <rect x="367.5" y="436.75" width="433" height="4.5" fill="black"/>
        <rect x="367.5" y="436.75" width="433" height="4.5" stroke="gray" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_61">
        <line x1="583.9721" y1="396.99996" x2="583.87946" y2="426.85005" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_62">
        <line x1="466.92135" y1="441.25" x2="467.14096" y2="471.10027" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_63">
        <line x1="706.332" y1="439" x2="706.0783" y2="471.1003" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
      <g id="Line_64">
        <line x1="467.2138" y1="1615" x2="467.2138" y2="1643.6" marker-end="url(#FilledArrow_Marker)" stroke="black" stroke-linecap="round" stroke-linejoin="round" stroke-width="1"/>
      </g>
    </g>
  </g>
</svg>